IV. Immunogenicity test
    9. HIV-1CRF01_AE based anti-HIV antibody detection
        Specimen
          - clotted blood/EDTA blood 3-5 ml.
          - serum
        Method
          - ELISA ( gp41, p24)
          - MPEIA ( gp41, p24)
          - IC ( gp41, RT, p24)
        Cost
          - sensitivity 99.6%
          - specificity 100%
        Result
          - under evaluation
     10. Neutralizing antibody test
        Specimen
          - clotted blood/EDTA blood 3-5 ml.
          - serum
        Method
          - Serial diluted serum with 50 TCID50 virus or Limiting diluted virus with four serum
          - single round cycle with RNA QT by real time PCR
        Cost
          - Contact NHRBC
        Result
          - within 1 month