II. HIV-1 genotype and biotype Characterization
       6. HIV-1 genotype ( subtype )
          Specimen
             - EDTA Blood 5 ml.
             - DBS ( Whatman )
          Method
             - HMA, MHA
             - gag, env   nucleotide sequencing
          Cost
             5,000-10,000 ฿/reaction
          Result
             - within 1 month
       7. HIV-1 biotype and phenotype
          Specimen
             - EDTA Blood 10 ml.
          Method
             - Co-culture
             - Infectivity test in PBMC, MT-2, primary macrophage, H9 cell, U87 CD4 CCR5,
               U87 CD4 CXCR4
          Cost
             - 20,000 ฿/reaction
          Result
             - within 1 month