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I. Diagnostic and monitory of HIV-1 infection
test kits
1. NHRBC
HIV-1 mutiplex PCR test kits
Specimen
-
Use only 0.5 ml EDTA Blood.
-
DBS ( Whatman )
Method
- 2 HIV genes ( pol and gag ) detection
simple
DNA lysate extraction for blood method.
Cost
-
200 ß/reaction
Result
-
within 1 week
2.
NHRBC HIV-1 RNA quantitative ( viral load ) by RT-PCR/ELONA
Specimen
-
EDTA at least 3 ml.
-
DBS ( Whatman )
Method
-
good for lab that have only DNA thermal cycles and ESISA reader
-
sensibility with low limit HIV-1 RNA detection at 10 copies/ml.
Cost
-
1,000 ß/reaction
Result
-
within 1 week
3.
NHRBC HIV-1 RNA quantitative by real time RT-PCR
Specimen
-
EDTA at least 3 ml.
-
DBS ( Whatman )
Method
-
good for lab that have real time PCR ex. LightCycler Roter Gene 3000,
TaqMan 48
-
sensibility with low limit HIV-1 RNA detection at 10 copies/ml.
Cost
-
1,000 ß/reaction
Result
-
within 3 days
4.
NHRBC HIV-1 drug resistant
Specimen
-
EDTA at least 3 ml.
-
DBS ( Whatman )
-
know viral load > 10,000 copies/ml
Method
-
HIV-1 pol necleotide sequencing
Cost
-
NRTI and/or NNRTI
2,000 ß/reaction
-
PI
2,000
ß/reaction
-
NRTI and/or NNRTI + PI 3,000
ß/reaction
Result
-
within 2 weeks
5.
Boosted p24 antigen QL ( for diagnostic ) and QT ( monitoring )
Specimen
-
EDTA Blood 3 ml.
Method
-
ELISA (ÚBioMerieux) with boosted step(ELAST)
Cost
-
QL 150
ß/reaction
-
QT 200
ß/reaction
Result
-
within 2 weeks
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